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Molecular Dynamics Inc personal densitometer si, model 375a
Personal Densitometer Si, Model 375a, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/personal+densitometer+si%2C+model+375a/personal+densitometer+si++model+375+a/pmc07040222-148-14-16
Average 90 stars, based on 1 article reviews
personal densitometer si, model 375a - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Electrophoresis:

Article Title: Directed-Mutagenesis of Flavobacterium meningosepticum Prolyl-Oligopeptidase and a Glutamine-Specific Endopeptidase From Barley
Article Snippet: .. After electrophoresis, each gel was subject to densitometric analysis using a Personal Densitometer SI, Model 375A (Molecular Dynamics), and the resulting images were analyzed using ImageJ software v 1.47. .. The HPLC separations were performed using a C8 reversed-phase analytical column (Zorbax 300SB-C8, Agilent Technologies) with 5 μm particle size and 30 nm microporous silica diameter (250 mm length, 4.6 mm inner diameter) and a C18 reversed-phase analytical column (Eclipse Plus C18, Agilent Technologies) with 5 μm particle size, 150 mm length, and 4.6 mm inner diameter.

Software:

Article Title: Factors influencing resistance of UV-irradiated DNA to the restriction endonuclease cleavage.
Article Snippet: DNA molecules of pUC19, pBR322 and X174 were irradiated by various doses of UV light and the irradiated molecules were cleaved by about two dozen type II restrictases.. The irradiation generally blocked the cleavage in a dose-dependent way.. In accordance with previous studies, the (A + T)-richness and the (PyPy) dimer content of the restriction site belongs among the factors that on average, cause an increase in the resistance of UV damaged DNA to the restrictase cleavage.

Article Title: Mapping the B-A conformational transition along plasmid DNA
Article Snippet: The gels were visualized under UV transilluminator (TM36 Model, Ultra-Violet Products Inc., San Gabriel, CA) and photographed with an orange filter. .. The negatives were quantified by densitometry using Personal Densitometer SI, Model 375A and ImageQuant software (Molecular Dynamics, Sunnyvale, CA). .. CD spectra were measured using a Jobin-Yvon Mark VI dichrograph in 0.1, 0.5 and 1 cm pathlength Hellma cells, and placed in a thermostatted holder.

Northern Blot:

Article Title: Protein synthesis and transcriptional inhibitors control N-methyl-N'-nitro-N-nitrosoguanidine-induced levels of APC mRNA in a p53-dependent manner.
Article Snippet: In the present study, we show that treatment of wild-type (p53) mouse embryonic fibroblast (MEF) cells with a DNA-alkylating agent, N-methyl-N'-nitro-N-nitrosoguanid ine ( M N N G ) , resul ted in increased levels of adenomatous polyposis coli (APC) mRNA compared to p53 gene-knocked out (p53-) MEF cells, indicating that p53 is required for APC expression after alkylation damage.. By using HCT-116 colon cancer cells (containing wild-type p53 gene) or p53 / _ MEF cells transfected with a pCMV-p53 overexpression plasmid [p53 / (CMV-p53)] , we show that p53 is a labile factor for APC gene expression, and that pretreating HCT-116 cells with a protein synthesis inhibitor, cycloheximide (CHX), inhibited MNNG-induced APC mRNA levels by inhibiting p53 protein synthesis.. The effect of CHX on p53 protein synthesis was reversible, as the withdrawal of CHX permitted p53 protein synthesis to resume with a concomitant increase in APC mRNA levels after MNNG treatment.

Western Blot:

Article Title: Protein synthesis and transcriptional inhibitors control N-methyl-N'-nitro-N-nitrosoguanidine-induced levels of APC mRNA in a p53-dependent manner.
Article Snippet: In the present study, we show that treatment of wild-type (p53) mouse embryonic fibroblast (MEF) cells with a DNA-alkylating agent, N-methyl-N'-nitro-N-nitrosoguanid ine ( M N N G ) , resul ted in increased levels of adenomatous polyposis coli (APC) mRNA compared to p53 gene-knocked out (p53-) MEF cells, indicating that p53 is required for APC expression after alkylation damage.. By using HCT-116 colon cancer cells (containing wild-type p53 gene) or p53 / _ MEF cells transfected with a pCMV-p53 overexpression plasmid [p53 / (CMV-p53)] , we show that p53 is a labile factor for APC gene expression, and that pretreating HCT-116 cells with a protein synthesis inhibitor, cycloheximide (CHX), inhibited MNNG-induced APC mRNA levels by inhibiting p53 protein synthesis.. The effect of CHX on p53 protein synthesis was reversible, as the withdrawal of CHX permitted p53 protein synthesis to resume with a concomitant increase in APC mRNA levels after MNNG treatment.



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Molecular Dynamics Inc personal densitometer si, model 375a
Personal Densitometer Si, Model 375a, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/personal+densitometer+si%2C+model+375a/personal+densitometer+si++model+375+a/pmc07040222-148-14-16
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